Which of the following accurately represents the site of action of hydrochlorothiazide?

A 45-year-old man presents to his primary care physician with a blood pressure of 160/90 mm Hg that has failed to drop substantially after initiation of lifestyle changes. The patient is subsequently placed on a low dose of hydrochlorothiazide, which lowers his blood pressure to 128/86 mm Hg. Which of the following accurately represents the site of action of hydrochlorothiazide?

(A) Chloride-binding sites on the cytoplasmic surface of the early distal tubule
(B) Chloride-binding sites on the luminal surface of the early distal tubule
© Chloride-binding sites on the luminal surface of the thick ascending limb of the loop of Henle
(D) Sodium binding sites on the basolateral membrane of the thick ascending limb of the loop of Henle
(E) Sodium-binding sites on the luminal surface of the early distal tubule