1.All the following are components of Collagen EXCEPT
A. Glycine
B. Proline
C. Lysine
D. Desmosine
Differences Between Collagen And Elastin
Collagen Elastin
Different types of
Collagen present Only one type exist.
Triple helix Structure
No triple helix instead
random coil
conformations
(Gly-X-Y) n repeating
structure
No (Gly-X-Y)n repeating
structure
Presence of
hydroxylysine
No hydroxylysine
Glycosylation present No Glycosylation
Intramolecular aldol
cross-links
Intramolecular
desmosine crosslinks
Presence of extension
peptides during
biosynthesis
No extension peptides
present during
biosynthesis
2.All the following can detect epigenetic modification except
A. HPLC
B. Methylation specific PCR
C. Bisulphite method
D. ChIP on Chip
Molecular Methods to Detect Epigenetic Modifications in the Genome
Traditional Sanger sequencing alone cannot detect epigenetic modification
- Methylation-specific PCR can detect DNA methylations
- DNA Chromatin Immuno Precipitation (ChIP) followed by Microarray hybdridisation analysis or Direct Sequencing(
ChIP- Chip or ChIP-Seq)detect histone modifications - Bisulphite Sequencing
- Methylation Sensitive Restriction Endonuclease Digestion
Principle of Bisulphite Sequencing
Sodium Bisulphite convert unmethylated Cytosine to Uracil, which function like thymine in base pairing.
But methylated Cytosines are protected from modification,hence remain unchanged
HPLC is a separatory technique or Purification technique