Diagnosis of beta thalassemia

Diagnosis of beta Thalassemia is established by:
A. NESTROFT Test
B. HbA1c estimation
C. Hb electrophoresis
D. Target cells in peripheral smear

Correct answer : C. Hb electrophoresis

NESTROFT Test – Naked Eye Single Tube Red Cell Osmotic Fragility Test – used for screening for Thalassemia
HbA1c – Used to assess long term glycemic control in diabetics (blood sugar control over past 3 months)
Target cells – A feature of thalassemia, but not diagnostic